Mustafa Abdullah Yilmaz
Dicle University, Turkey
Title: Secondary metabolic profile of 14 Achillea species by LC-MS/MS, LC-MS IT-TOF and investigation of their biological activities
Biography
Biography: Mustafa Abdullah Yilmaz
Abstract
In this study, secondary metabolic profile of ethanol extracts of 4 different Achillea species (A. Arabica, A. santalinoides, A. vermicularis) were determined using LC-MS/MS. A comprehensive LC-MS/MS method validation was developed for the qualitative and quantitative analysis of 37 phytochemicals including 15 phenolic acids, 17 flavonoids, 3 nonphenolic organic acids, 1 phenolic aldehyde and 1 penzopyrane. The analytes were quantified by a triple quadrupole mass spectrometer working in multiple reaction monitoring (MRM) mode. The fragmentation patterns of the studied compounds using ESI and collision-induced dissociation (CID) techniques are reported. The performance properties of the analytical method were determined by using standard solutions, spiked and non-spiked samples. Within the context of method validation, linearity, trueness (recovery), precision (repeatability and reproducibility, LOD and LOQ and expanded uncertainty (at 95% confidence level (k=2)) were determined. Afterwards, different parts of 4 different Achillea species were analysed and their phytochemical constituents were quantified by this method.
