Industrial Pharmacy & 5^th Global Pharmacovigilance Summit

Biography

Biography: Manish Sharma

Abstract

A comparative QSAR study through Hansch analysis on PPAR α and γ agonists was carried out on following three series: 1) 2-Alkoxydihydrocinnamates. -logEC50=2.053(±0.899)R1Vw-1.921(±0.625)R2Vw+6.476(±0.375) n=12, r=0.95, s=0.18, F=41.37, R2=0.90, R2adj=0.88, RMSE=0.38, Q2=0.81 2) Azaindole-α-alkyloxyphenylpropionic acids. -logEC50= -0.96(±0.472)RVw+0.847(±0.344)I1+0.495(±0.249)I2+6.476 n=18, r=0.94, s=0.19, F=32.58, R2=0.88, R2adj=0.85, RMSE=0.20, Q2=0.78 3) Oxime ethers of α-acyl-β-phenylpropanoic acids. -logEC50=11.344(±6.549)CMR-0.415(±0.255)CMR2-68.072(±42.133) n=15, r=0.90, s=0.26, F=24.84, R2=0.82, R2adj=0.80,RMSE=0.41, Q2=0.73 On the basis of internally and laterally validated QSAR models and results of QSAR equation on oxime ethers of α-acyl-β-phenylpropanoic acids, scaled calculated optimum molar refractivity (CMRo) value of 14.3 for molecules was required for maximum agonistic activity. Using this clue, some oxime ethers of α-acyl-β-phenylpropanoic acids were proposed which were within the probability density derived applicability domain. The structural effects of ligand binding were examined on the basis of hydrogen bond interactions and binding energies in the final complexes obtained from molecular docking simulations. Compound RM-KT-01 was found to possess optimum calculated activity, passed Lipinski’s “rule of five” for oral absorption and lacked toxicity (mutagenicity, carcinogenicity, teratogenicity and embryotoxicity predicted by PASS). The derivative was synthesized and characterized by their physicochemical data and spectral analysis (F.T.I.R, 1H N.M.R., Elemental analysis and Mass spectroscopy). The synthesized compound RM-KT-01 (1) was screened for human PPAR (hPPAR) α agonistic activity on full length PPARα receptor transfected in HepG2 cells. The in vitro PPAR α agonistic activity (EC50) of synthesized compound was reported to be 78 nM. The in vitro activity suggests relevancy of presence of phenyl carboxamide group at one end and n-butyl group attached with phenylpropanoic acid chain of oxime ether of α-acyl-β phenylpropanoic acid. The result of transactivation assay also suggests that RM-KT-01 (1) is PPAR α selective and shows no activity on PPAR γ and PPAR δ.