Joint International Conference and expo on Industrial Pharmacy & 5^th Global Pharmacovigilance Summit April 28-29, 2016 Dubai, UAE

Hamdi TEMEL is a full professor since 2008 at Dicle University, Diyarbakır, TURKEY. He is dean of the Faculty of Pharmacy in Dicle University. His research areas are synthesis and characterization of biologically active compounds, antioxidant studies..etc. He has published more than 90 papers in reputed journals.

Being the most indigenious economic plants of Anatolia, Achillea genus is widespread all over the world and in literature its species have been investigated to possess several biological activities including cytotoxicty. In the present study, cytotoxic activities of chloroform-methanol extracts (1:1) of underground and overground parts of four Achillea species (A. monocephala, A. nobilis, A. goniocephala and A. sintenisii ) on HeLa (Human Cervical Carcinoma Cell Line) cell lines at concentrations 50, 100 and 250 µg/mL were investigated. The cytotoxic activities were tested using a real-time analyzer (xCELLigence). The xCELLigence system was used with the disposable E-plate 96 for the measurements of solvent extracts, controls and medium. The impedance difference based measurements were caused by the cells attached to the E-Plate 96. Fortunately, all of the extracts showed very high cytotoxic activity depending on the concentration. The lower the extract concentration the lower the cytotoxic activity. The highest cytotoxic activities were shown by underground extracts of A. monocephala (-0.0142) and A. goniocephala (-0.0119).

Hamdi TEMEL is a full professor since 2008 at Dicle University, Diyarbakır, TURKEY. He is dean of the Faculty of Pharmacy in Dicle University. His research areas are synthesis and characterization of biologically active compounds, antioxidant studies..etc. He has published more than 90 papers in reputed journals.

Achillea L. (Asteraceae) is a widely distributed medicinal plant and represented by 140 species in the world. Achillea L. species are commonly used in Turkish Traditional Medicine for the treatment of wounds, bleedings, headache, inflammation, pains, spasmodic diseases, flatulence and dyspepsia and hemorrhoids for years. In this study, methanol extracts of underground and overground parts of 4 different Achillea species (A. monocephala, A. nobilis, A. goniocephala and A. sintenisii ) were investigated in terms of their antioxidant activities by using DPPH free radical scavenging assay, ABTS radical cation decolorization assay, β-carotene lipit peroxidation test system and CUPRAC (copper reduction capacity) methods. For DPPH A. nobilis-underground (IC50: 12,23±0,24), for ABTS A. monocephala-underground (<10) and A. nobilis-underground (<10) and for CUPRAC A. nobilis underground (3.675±0,130) extracts were the most active extracts for the studied methods. And for β- carotene lipid peroxidation test system all of the studied species were low or moderately active. Moreover, total phenolic content (A. nobilis-underground (282,97±3,14 μg pyrocatechol equivalent/mg extract)) and total flavonoid content (A. nobilis-overground (24.77±0.71 μg quercetin eqivalent /mg extract)) of the studied species were investigated. According to the anticholinesterase activity results the highest activity in both butyrylcholinesterase and acetylcholinesterase enzyme inhibition was shown by overground extract of A. monocephala (39.73±1.51 and 19.60±0.44 respectively).

Mustafa Abdullah YILMAZ is an assistant professor at Dicle University, Faculty of Pharmacy, Diyarbakır, TURKEY. He is also in charge of ther mass spectrometry and chromatography unit in Dicle University Science and Technology Application and Research Center (DUBTAM). His research areas are (high resolution) mass spectrometry and chromatography (LC-MS/MS, GC-MS, LC-MS IT-TOF), plant metabolomics, analytical method validation ..etc. He has published 10 papers in reputed journals.

In this study, secondary metabolic profile of ethanol extracts of 4 different Achillea species (A. Arabica, A. Santalanoides, A. Vermicularis) were determined using LC-MS/MS. A comprehensive LC-MS/MS method validation was developed for the qualitative and quantitative analysis of 37 phytochemicals including 15 Phenolic acids, 17 flavonoids, 3 nonphenolic organic acids, 1 phenolic aldehyde and 1 penzopyrane. The analytes were quantified by a triple quadrupole mass spectrometer working in multiple reaction monitoring (MRM) mode. The fragmentation patterns of the studied compounds using ESI and collision-induced dissociation (CID) techniques are reported. The performance properties of the analytical method were determined by using standard solutions, spiked and non-spiked samples. Within the context of method validation, linearity, trueness (recovery), precision (repeatability and reproducibility, LOD and LOQ and expanded uncertainty (at 95% confidence level (k=2)) were determined. Afterwards, different parts of 4 different Achillea species were analysed and their phytochemical constituents were quantified by this method

Turgumbayeva A A did PhD from Asfendiyarov S. Kazakh National Medical University, School Pharmacy, for specialty "Technology of pharmaceutical production”. After graduating from university, she worked as an intern researcher in LLP "Company Ak Mai". In 2010, he did Master’s degree on specialty "Chemical technology of organic substances" Kazakh National University. Al-Farabi. In 2012, he entered PhD Doctoral on a specialty "Technology of pharmaceutical production” the Kazakh National Medical University. S. Asfendiyarov, School Pharmacy. She has more than 35 scientific papers, 3 articles with impact factor, 1 article in Scopus, 4 Elaboration and approval of the PAND for the preparation substances. She has passed scientific training in Xinjiang Technical Institute of Physics and Chemistry in Urumqi (China) for 1 month (June 2011), University of Mississippi, Oxford (USA) for 3 months (from 17.10.2013 till 01.13.2014), the Armenian Medical University, Yerevan, as well as the Lublin Medical University, Lublin (Poland).

Carthamus tinctorius L. is commonly known as safflower C. tinctorius extracts and oil are important in drug development with numerous pharmacological activities in the world. This plant is cultivated mainly for its seed, which is used as edible oil. It is also known as "Safflower" and has been a subject of several chemical and pharmacological studies. Carthamus tinctorius L. is an important medicinal plant of Kazakhstan. It is used especially for wound healing, antimicrobial, anti-inflammatory, cytotoxic, spasmolytic, jaundice and as an antispasmodic. Chemical studies have the presence of various classes of compounds, the main being flavonoids, quinones, coumarines, volatile oil, free fatty acids and amino acids. The extract of this plant as well as pure compounds isolated from it, have been demonstrated to possess multiple pharmacological activities. In this review, we have explored the Phytochemistry and pharmacological activities of Carthamus tinctorius L. as well as highlight its activity properties as a medicinal agent. An ointment of essential oil from safflower flowers was grown in Kazakhstan for creating ointment of flowers safflower (Carthamus tinctorius L.) optimum composition of the excipients. So several models were created ointment like based - emulsion, a slurry, combined with application of various excipients - sunflower oil, glycerol, paraffin oil, lanolin, etc., Emulsifiers - Tween-80, T-2 and others. The most efficient composition of the technological parameters was ointment base with the following composition: essential oil obtained from the flowers of safflower 9.0 g., sunflower oil 40 ml., T-2 5 g., Purified Water 46.8 ml., Oleum menthae piperitae 0.2 ml. An experimental industrial series of ointment based medicinal plant C. tinctorius L. studies was obtained in animals (rabbits, guinea pigs, white rats) which showed harmless and good tolerability.

Swati Khare has completed her Master’s in Pharmacy (MPharma) in Pharmaceutics (Honors) from College of Pharmacy, IPS Academy, Indore (M.P.) in 2012. Currently she is working with IIST College Indore as Assistant Professor. Earlier she had worked with Cyano Pharma Pvt. Ltd. Indore as Q.A. Officer. She has presented poster on “Innovative pharmacy & Pharmaceutical Science” in Bhopal and paper presentation during Technopharma event held in S.I.R.T. Bhopal in April 2012.

Truncate infections caused by Helminthes, or parasitic worms, affect more than two billion people worldwide. Looking at this situation, an attempt has been made to formulate a novel drug delivery system known as medicated chewing gum containing masticatory gum base with pharmacologically active ingredient Diethylcarbamazine citrate (used as a first-line agent for control and treatment of Lymphatic filariasis and for therapy of tropical pulmonary eosinophilia caused by Wuchereria bancrofti and Brugia malayi). Optimized formulations of medicated chewing gum with varying concentration of gum base were formulated. Evaluation parameter like texture analysis (hardness, firmness and springiness test) is carried out by texture analyzer apparatus (TAXT plus). Improved essentials of casting & in-vitro release profile of drug in saliva was obtained by formulation Fc3 (96.2%). Buccal absorption studies showed that 39.2% of drug absorbed within one minute when available to buccal mucosa at pH 5.5, commensurate with explain diethylcarbamazine citrate- medicated chewing gum (DEC-MCG) can be considered as better formulation for buccal drug delivery system in which drug is absorbed buccally and reaches the systemic circulation via jugular vein.

Saritha Marella has done Post-graduation in the Department of Biochemistry, Sri Venkateswara University, Tirupati in 2002. She was a member in the Society of Biological Chemists from 2000-2002 and worked as Lecturer on contract basis for teaching Under-graduate and Post-graduate students from 2002-2004 and from 2005-2007 respectively. She worked as JRF in DST-PURSE funded by Department of Science and Technology, New Delhi for 3 years during her Doctoral work and published work in 5 international journals. She also presented papers (Oral and Poster presentation) in many international symposia and conferences. She also acted as a member in the Organizing Committee and as a Resource person in some of the national conferences.

The study was aimed to evaluate the protective effects of a novel anti-hyperglycemic “Mcy protein” (17 KDa) isolated from the fruits of Momordica cymbalaria in streptozotocin induced- diabetes rat model. The protein was purified by Ammonium sulfate precipitation, Gel filtration and High Performance Liquid Chromatography (HPLC) and further biochemically characterized. Fasting blood glucose and, serum and tissue lipid levels were measured along with biochemical analysis for hepatic and renal function tests. Mcy protein significantly reduced the fasting blood glucose and, serum as well as tissue lipid levels (p<0.05), besides normalizing the levels of liver and kidney function markers in the treated diabetic rats when compared to the diabetic controls. The in vitro studies are promising to say that Mcy has good inhibitory actions on both α-glucosidase and aldose reductase enzyme activities with an IC50 224 µg/ml and 164 µg/ml respectively. This proves to be effective in preventing development of diabetic complications like severe post prandial hyper glycemia (PPHG) and cataract and thus preventing further deleterious effects like neuropathy, nephropathy and retinopathy. Structural studies using circular dichroism spectroscopy indicate that MCP contains 84.27% of α-helix and 1.24% of β strand. Thus MCP is an effective alternative complementary medicine and beneficial therapeutic agent for diabetes mellitus.